Selection of Kanamycin-Resistant Transformants: Isolation and propagation of E. coli carrying kanamycin-resistance plasmids at 30 µg/mL.

Colony Isolation and Streaking: Streaking for single colonies of Kan^R strains for downstream cloning and verification.

High-Density Library Plating: Arraying large genomic or plasmid libraries on 245×245 mm plates for efficient screening.

Replica Plating: Transferring colonies to secondary selective media for phenotype/genotype confirmation.

CRISPR and Allelic Exchange Selection: Selecting edited clones using kanamycin resistance markers.

Transposon Mutagenesis Screens: Selecting insertion mutants carrying kanamycin cassettes.

Conjugation/Mating Selections: Recovering Kan^R transconjugants after bacterial mating experiments.

Expression Control During Cloning: 1% glucose suppresses basal lac-based expression to stabilize toxic or burdening constructs in E. coli.

CFU Enumeration of Kan^R Cells: Quantifying viable counts of antibiotic-resistant populations.

Colony Lifts and Screening Assays: Performing membrane lifts for hybridization or activity screens from Kan^R colonies.