Selection of Kanamycin-Resistant Transformants: Isolating recombinant E. coli colonies carrying kanR plasmids.

Plasmid Maintenance: Routine propagation of kanamycin-selectable plasmids in E. coli streaks and lawns.

Protein Expression Workflows: Culturing recombinant E. coli harboring kanR expression vectors prior to induction.

Library Screening and Colony Picking: High-density selection on 245 mm plates for transformant libraries and automated picking.

CRISPR/Cas Editing Selection: Enrichment of edited E. coli clones using kanamycin markers.

Conjugation and Transposon Mutagenesis: Selecting kanR recipients or insertions in E. coli and related Gram-negative bacteria.

Replica Plating: Large-format replica plating for phenotype and antibiotic sensitivity screens under kanamycin selection.

Strain Isolation and QC: Streaking for single colonies and verifying stability of kan-marked strains.

Blue-White/Reporter Screening: Colony screening on kan-plates when supplemented with X-gal/IPTG or appropriate reporters.

Agrobacterium Vector Maintenance: Selection of kanR binary vectors in Agrobacterium tumefaciens when kan25 is appropriate.