Antibiotic Selection and Plasmid Maintenance: Selects and maintains plasmids bearing gentamicin, kanamycin, or tetracycline resistance in E. coli.

Co-selection of Multiple Plasmids: Simultaneously maintains distinct plasmids with Gent/Kan/Tet markers in cloning and expression strains of E. coli.

Blue-White Screening (lacZα Complementation): Differentiates recombinant (white) from non-recombinant (blue) colonies using X-Gal/IPTG in E. coli.

Screening of Cloning Outputs: Rapidly evaluates ligation, Gibson, or Golden Gate assemblies via resistance and blue-white readout in E. coli.

Bacterial Two-Hybrid and Multi-Vector Systems: Enables selection and colorimetric reporter readouts for multi-plasmid assays in E. coli.

Library Construction and Transposon Screens: Builds and screens genomic or transposon libraries under multi-antibiotic pressure with lacZ-based reporting in E. coli.

Conjugation/Triparental Mating Selections: Selects recipients carrying transferred plasmids with different resistances and lacZ reporters, typically in E. coli.

Replica Plating and Plasmid Stability QC: Confirms genotype–phenotype linkage and assesses plasmid segregation stability under combined Gent/Kan/Tet in E. coli.