Selection of Kanamycin-Resistant Transformants: Isolates colonies harboring kanR plasmids after transformation of E. coli.

Plasmid Maintenance: Ensures stable propagation of kanamycin-selectable plasmids in E. coli.

Colony Screening: Supports colony PCR, miniprep, and sequencing from kan-selected E. coli colonies.

CFU Enumeration: Enables counting and titration of kan-resistant E. coli populations.

Expression Screening: Screens recombinant protein expression from kanR vectors in E. coli.

Transformation Efficiency Testing: Benchmarks competent cells using kanR reporter plasmids in E. coli.

Replica Plating and Stability Testing: Verifies plasmid retention and phenotype stability under kanamycin selection in E. coli.

Conjugation/Mating Selections: Selects transconjugants carrying kanamycin resistance markers in E. coli crosses.

Library Construction and Maintenance: Builds and preserves plasmid/genomic libraries requiring kan selection in E. coli.

Strain Banking and Passaging: Short-term storage and routine subculture of kan-resistant E. coli strains.