Antibiotic Selection of Transformants: Isolation of kanamycin-resistant colonies after plasmid transformation in Escherichia coli.

Plasmid Maintenance: Stable propagation of KanR plasmids in cloning and protein expression strains of Escherichia coli.

Colony Screening: Picking colonies for colony PCR, Sanger sequencing, or miniprep/restriction analysis under kanamycin selection.

Streaking for Single Colonies: Purification of KanR strains by streaking and re-isolation on selective LB agar.

CFU Enumeration: Viable count assays and titering of kanamycin-resistant bacteria such as Escherichia coli and Bacillus subtilis (KanR).

Conjugation/Transduction Selection: Selecting recipients or transductants carrying KanR markers after mating or phage transduction.

Genome Editing Verification: Selection and maintenance of strains with kanamycin cassette integrations or replacements.

Strain Archiving: Short-term storage and stab cultures of KanR strains on solid medium.

Library Screens: Selection of pooled or arrayed mutant libraries (e.g., transposon or CRISPRi libraries) using kanamycin resistance.

Heterologous Expression Workflows: Maintaining expression constructs with KanR in hosts like Escherichia coli and Bacillus subtilis (KanR).