Selection of Kanamycin-Resistant Transformants: Isolates colonies after introducing KanR plasmids into Escherichia coli.

Plasmid Maintenance and Amplification: Propagates plasmids carrying a kanamycin resistance marker in E. coli.

Colony Isolation and Streaking for Purity: Obtains single colonies and maintains pure KanR strains.

Colony PCR and Sequencing Prep: Provides colonies for insert verification by PCR and Sanger sequencing.

CFU Enumeration and Transformation Efficiency: Quantifies colony-forming units post-transformation with KanR constructs.

Cloning and Construct Screening: Screens recombinant clones harboring kanamycin-selectable vectors.

CRISPR/Recombineering Selection: Selects edits or insertions marked with a kanamycin resistance cassette in E. coli.

Strain Maintenance and Glycerol Stock Prep: Picks verified KanR colonies for long-term archiving.

Protein Expression Workflow: Maintains expression plasmids with KanR in production strains of E. coli.

Conjugation/Mating Selection: Selects recipients or transconjugants acquiring kanamycin-marked elements.