Antibiotic Selection of Transformants: Plate transformed E. coli on LB-Amp (100 µg/ml) to select colonies carrying bla-encoded, ampicillin-resistant plasmids.

Cloning and Plasmid Maintenance: Grow and maintain plasmid-bearing E. coli colonies prior to miniprep or downstream workflows.

Colony Screening and PCR: Pick antibiotic-selected colonies for colony PCR, restriction analysis, or sequencing confirmation.

Streaking for Single Colonies: Streak mixed or liquid cultures to isolate single ampicillin-resistant colonies.

Transformation Efficiency and CFU Enumeration: Quantify transformation performance by counting colony-forming units on selective plates.

Plasmid Library Construction and Screening: Maintain and screen combinatorial or genomic libraries under ampicillin selection in E. coli.

Pre-culture Selection for Protein Expression: Select expression strains harboring AmpR plasmids before induction in liquid media.

Replica Plating and Stability Testing: Assess plasmid retention, segregation stability, or antibiotic resistance profiles by replica plating.

Conjugation/Mating Selection: Select transconjugants when transferred plasmids confer ampicillin resistance in E. coli.

Stock Preparation: Pick verified colonies from selective plates to establish glycerol stocks for long-term storage.