Dual-Plasmid Selection in Escherichia coli: Selects co-transformants carrying AmpR (carbenicillin 100 µg/mL) and CamR (chloramphenicol 34 µg/mL) on LB agar.

Transformation and Cloning Workflows: Isolates recombinant E. coli colonies after ligation/Gibson assembly requiring simultaneous AmpR and CamR selection.

Maintenance of Co-Expressed Systems: Sustains dual plasmid expression constructs in E. coli for protein production under combined antibiotic pressure.

BAC/Plasmid Co-Maintenance: Supports chloramphenicol-resistant BACs with an AmpR helper or expression plasmid in E. coli.

CRISPR/Cas Workflows: Selects E. coli cells harboring separate Cas and guide/donor plasmids marked with AmpR and CamR.

Library Screening and Colony Picking: Enables high-throughput picking of dual-resistant E. coli colonies for downstream colony PCR and sequencing.

Plasmid Stability Assays: Evaluates retention of AmpR- and CamR-marked plasmids in E. coli under dual-antibiotic selection.

Conjugation/Mating Selection: Recovers E. coli transconjugants that acquire both resistance markers after transfer events.

Reporter and Pathway Engineering: Maintains multi-component metabolic or reporter circuits split across AmpR and CamR plasmids in E. coli.

Quality Control of Resistance Cassettes: Verifies correct insertion/expression of beta-lactamase and chloramphenicol acetyltransferase markers in E. coli.