Antibiotic Selection of Transformants: Selects kanamycin-resistant colonies after transformation in Escherichia coli using LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar) with 50 µg/mL Kanamycin.

Plasmid Maintenance and Expression: Maintains kanR plasmids and stable expression constructs in E. coli under selective pressure.

Colony Screening and Picking: Supports growth of discrete kanamycin-resistant colonies for downstream PCR, minipreps, or sequencing.

High-Throughput Screening: 8-well 86×128 mm format streamlines parallel assays, strain comparisons, and construct verification.

Replica Plating and Strain Validation: Confirms genotype/phenotype linkage by transferring colonies onto selective LB-kan plates.

Conjugation and Transposition Selection: Recovers transconjugants or transposon insertions carrying kanamycin resistance in E. coli and related hosts.

CRISPR/Cas Workflows: Selects edited clones harboring kanR markers or helper plasmids during genome engineering in E. coli.

Bacterial Library Maintenance: Preserves pooled or arrayed kanR libraries and barcoded collections in E. coli for downstream analysis.

Transformation Efficiency Testing: Quantifies competent cell performance by plating kanR control plasmids.

Agrobacterium Workflows: Selects kanR plasmids in Agrobacterium tumefaciens prior to plant transformation steps.