Transformant Selection: Selects colonies harboring bla-carbenicillin–resistant plasmids after chemical or electroporation transformation in Escherichia coli.

Plasmid Maintenance: Maintains selective pressure for plasmid propagation in E. coli cultures.

Colony Screening (Blue-White or Reporter-Assisted): Supports screening of recombinant clones when supplemented with X-gal/IPTG or appropriate reporters.

Colony PCR: Enables rapid PCR verification directly from antibiotic-selected colonies.

CFU Enumeration: Quantifies viable, antibiotic-resistant cells via colony forming unit counts.

Streaking and Isolation: Obtains single colonies for clonal isolation and purity checks under beta-lactam selection.

Starter Culture Picking: Provides antibiotic-selected colonies for liquid culture inoculation and downstream plasmid prep.

Protein Expression Workflow: Prescreens carbenicillin-resistant expression strains of E. coli prior to induction.

Glycerol Stock Preparation: Generates verified clonal isolates for long-term storage following selection.

Strain Verification: Differentiates resistant vs. sensitive backgrounds during strain construction in E. coli.