Selection and Maintenance of Plasmids: Isolating and propagating kanamycin-resistant clones in E. coli and other bacteria using 50µg/mL kanamycin.

Bacterial Transformation Screening: Selecting successful transformants after chemical or electroporation methods in E. coli, Bacillus subtilis, and related species.

Colony Isolation and Purification: Streaking and re-streaking to obtain pure kanamycin-resistant colonies from mixed cultures.

CFU Enumeration Under Selection: Quantifying viable cells carrying kanamycin-resistance markers via selective plating.

Conjugation and Transposon Mutagenesis: Selecting transconjugants or transposon insertions that confer kanamycin resistance.

Genome Editing Workflows: Selecting CRISPR-edited strains or recombinants incorporating kanamycin resistance cassettes.

Maintenance of Knockout/Tagged Strains: Propagating strains with kanamycin resistance markers integrated into the chromosome.

Colony PCR and Downstream Cloning: Picking kanamycin-resistant colonies for PCR verification and plasmid prep.

Reporter and Screening Assays: Performing phenotype or reporter assays on selective plates; compatible with X-gal/IPTG when required for blue-white screening.

Strain Archiving: Short-term storage and recovery of validated kanamycin-resistant clones.