Selection of Kanamycin-Resistant Transformants: Isolation of recombinant colonies after transformation of Escherichia coli or other kan-resistant hosts using LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar) with 25 µg/mL kanamycin.

Plasmid Maintenance and Propagation: Stable selection and passage of plasmids carrying kanamycin resistance cassettes in strains such as Escherichia coli, Agrobacterium tumefaciens, and Pseudomonas putida.

Colony Screening and Picking: Growth of individual colonies for downstream colony PCR, restriction analysis, or sequencing under kanamycin selection.

Library Construction and Screening: Selection of genomic, cDNA, or mutagenesis libraries cloned into kanamycin-resistant vectors.

Protein Expression Strain Selection: Maintenance of expression hosts (e.g., T7-based systems) harboring kanamycin-selectable plasmids prior to induction.

CRISPR/Cas and Recombineering Workflows: Selection of edited clones using kanamycin markers during genome engineering in Escherichia coli and related bacteria.

Conjugation and Transposon Mutagenesis: Post-mating selection of recipients or insertion events using kanamycin resistance.

CFU Enumeration Under Selection: Quantification of viable, kan-resistant cells and assessment of transformation efficiency or contamination.

Replica Plating and Strain Authentication: Verification of genotype/phenotype linkage and maintenance of kanamycin-selectable traits.

Recovery from Glycerol Stocks and Streak Isolation: Revival and purification of kan-resistant strains for subsequent experiments.