Antibiotic Selection of bla+ Clones: Selects and maintains Escherichia coli harboring ampicillin/carbenicillin-resistance plasmids (100 µg/mL carbenicillin) on LB agar (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl, 1.5% agar).

Cloning and Transformation Screening: Recovery and screening of transformants carrying carbenicillin-selectable plasmids after heat-shock or electroporation.

Streaking for Isolation: Single-colony purification of recombinant E. coli strains to ensure clonal populations.

Colony PCR and Insert Verification: Picking antibiotic-selected colonies for colony PCR, restriction analysis, or sequencing.

CFU Enumeration Under Selection: Quantifying transformation efficiency or viable counts of carbenicillin-resistant cells.

Blue-White Screening (With Supplements): α-complementation screening when supplemented with X-Gal/IPTG on carbenicillin plates.

Pre-culture Starter Colony Selection: Picking verified colonies to start liquid cultures for minipreps, midipreps, or protein expression.

Reduced Satellite Colonies vs Ampicillin: Using carbenicillin to minimize satellite colony formation around β-lactamase+ colonies.

High-Throughput Colony Handling: Rectangular 86×128 mm format suited for robotics, replica plating, and automated colony picking.

Conjugation/Strain Construction Selection: Selecting recipients/donors carrying bla-marked plasmids in genetic crosses.