Antibiotic Selection of Transformants: Selects bla-bearing, carbenicillin-resistant colonies after transformation of E. coli.

Plasmid Maintenance and Cloning: Maintains recombinant plasmids in E. coli using 50 µg/mL carbenicillin for stable selection.

Colony Screening (Colony PCR/Sequencing): Enables picking single colonies for colony PCR, Sanger prep, and downstream validation.

Blue-White Screening: Supports lacZα-complementation (with X-gal/IPTG added) to distinguish recombinant clones in E. coli.

Transformation Efficiency and CFU Enumeration: Plates heat-shock/electroporation mixtures to quantify CFUs under carbenicillin selection.

Streaking for Single Colonies: Isolates clonal E. coli populations for starter cultures and glycerol stock preparation.

Protein Expression Workflow Setup: Plates expression strains (e.g., BL21 derivatives) carrying carbenicillin-selectable plasmids prior to induction.

Library Construction and Maintenance: Sustains plasmid libraries in E. coli under β-lactam selection to preserve diversity.

Plasmid Recovery (Miniprep Source): Provides single colonies for inoculating carbenicillin-supplemented liquid cultures before miniprep.

General CFU/Viability Checks: Assesses viability of carbenicillin-resistant E. coli strains on standard LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar) with 50 µg/mL carbenicillin.