Plasmid Selection and Maintenance: Selects and maintains AmpR plasmid-bearing Escherichia coli using 50 µg/mL ampicillin on LB agar (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar).

Transformation Screening: Recovers and screens heat-shock or electroporation transformants of Escherichia coli for ampicillin resistance.

Colony Isolation and Purification: Streaks single colonies to purity prior to liquid culture or glycerol stock preparation.

Colony PCR: Provides colonies as DNA templates to verify inserts or vector presence in AmpR clones.

Blue-White Screening (with X-Gal/IPTG): Enables α-complementation-based screening of recombinant clones on Amp plates when supplemented.

Expression Construct Screening: Screens recombinant Escherichia coli strains harboring AmpR expression vectors before induction in liquid culture.

Library Cloning and Subcloning: Selects ampicillin-resistant colonies during construction and refinement of DNA libraries.

CFU Enumeration: Quantifies viable counts of AmpR Escherichia coli following transformation or outgrowth.

Antibiotic Resistance Verification: Confirms functional AmpR markers and assesses loss/retention of plasmids under selection.

Bacterial Conjugation/Mating Selection: Selects transconjugants carrying AmpR plasmids on LB agar with ampicillin.