Antibiotic Selection of Transformants: Selects colonies carrying chloramphenicol-resistance plasmids in E. coli at 30 µg/mL.

Plasmid Maintenance and Propagation: Maintains CmR cloning/expression vectors (e.g., pACYC-derived) in E. coli strains.

Post-Cloning Screening: Screens recombinant colonies after ligation, Gibson, or Golden Gate assembly into CmR vectors in E. coli.

Colony Isolation and CFU Enumeration: Streaks, isolates single colonies, and performs CFU counts under chloramphenicol selection.

Library Plating: Plates genomic, cDNA, or mutant libraries constructed on chloramphenicol-resistant backbones in E. coli.

Multi-Plasmid Co-Selection: Supports co-transformation with distinct antibiotic markers including chloramphenicol in E. coli.

BAC/PAC/Fosmid Work: Selects and maintains large-insert constructs with chloramphenicol markers in E. coli.

Replica Plating and Stability Assays: Assesses plasmid retention and strain stability under chloramphenicol pressure in E. coli.

Strain or Vector Verification: Confirms chloramphenicol resistance phenotypes in engineered E. coli strains.

Glycerol Stock Preparation: Picks verified chloramphenicol-resistant colonies for long-term storage.