Custom Order

We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

L1939

LB Agar Plates with Carbenicillin-100, Kanamycin-25. 100mm, 20 Plates per Sleeve, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

100mm

Item Count

20 Plate(s)

Price per Item

$120.00

Fill Volume

25 mL

COA
COA

L1939

LB Agar Plates with Carbenicillin-100, Kanamycin-25. 100mm, 20 Plates per Sleeve, Sterile.

Quantity

Minimum is set to 8 for this product*

scroll

Description
Formulation
Common Applications
Storage

LB Agar Plates with Carbenicillin-100 and Kanamycin-25 are solid nutrient media for cultivation and dual-antibiotic selection of E. coli strains. The inclusion of 100 µg/mL carbenicillin and 25 µg/mL kanamycin enables selection of transformants carrying beta-lactam and aminoglycoside resistance markers during routine streaking and colony isolation.

  • 1.0% Tryptone
  • 0.5% Yeast Extract
  • 1.0% Sodium Chloride
  • 1.5% Agar
  • 100µg/mL Carbenicillin
  • 25µg/mL Kanamycin

  • Dual-antibiotic selection of transformants: Isolating recombinant Escherichia coli carrying plasmids with beta-lactam (bla) and kanamycin (nptII/aph) resistance markers.

  • Co-transformation and plasmid co-maintenance: Selecting Escherichia coli clones harboring two compatible plasmids requiring carbenicillin and kanamycin.

  • Cloning and assembly screening: Identifying correct colonies after Gibson, Golden Gate, or ligation under dual selection pressure.

  • Library construction and maintenance: Preserving diversity in combinatorial or mutagenesis libraries that use both resistance markers.

  • Plasmid stability assays: Evaluating segregational stability of single or multiple plasmids in Escherichia coli with continuous dual selection.

  • CFU enumeration post-transformation: Quantifying transformation efficiency of dual-resistant constructs in Escherichia coli.

  • Pre-expression strain verification: Confirming correct antibiotic resistance in expression strains before induction in liquid culture.

  • Chromosomal integration workflows: Selecting strains with a kanamycin-tagged genomic insertion plus a carbenicillin-resistant helper or expression plasmid.

  • Replica plating and marker verification: Validating presence of dual resistance markers in cloned strains of Escherichia coli.

  • Contamination control: Restricting growth to intended dual-resistant Escherichia coli strains on LB agar.

Storage Temperature
Refrigerated
Shelf Life
60 days
Shows related citations for this product
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA
We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button