Blue-White Screening of Recombinant Clones: α-complementation-based selection of inserts in lacZα plasmids using E. coli.

Bacterial Transformation Screening: Rapid identification of transformants carrying inserts after chemical or electroporation methods in E. coli.

Colony Picking and Clone Isolation: Distinguish blue (non-recombinant) from white (recombinant) colonies for downstream plasmid prep and sequencing.

Library Construction and Screening: cDNA/genomic library screening via lacZα disruption to enrich for insert-containing clones in E. coli.

TA/TOPO Cloning Workflows: Visual selection of recombinant inserts in lacZα-based TA or TOPO vectors in E. coli.

Phage Plaque Color Screening: Blue/white plaque discrimination for recombinant M13 or λ-based lacZα phage systems.

lacZ Reporter Assays: Qualitative assessment of β-galactosidase activity and promoter induction using IPTG and X-Gal in E. coli.

Competent Cell QC: Validate α-complementation efficiency and transformation performance of E. coli competent cells.

CRISPR/Edit Validation with lacZ: Screen for frameshift or insertion events disrupting lacZα, yielding white colonies in E. coli.

Synthetic Biology Part Screening: Rapid characterization of lac-based regulatory elements and cloning constructs in E. coli.