Custom Order

We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

L1919

LB Agar Plates with Gentamicin-7, Kanamycin-50, Tetracycline-10, Bluo-gal-100, IPTG-40. 100mm, 20 Plates/Sleeve, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

100mm

Item Count

20 Plate(s)

Price per Item

$91.00

Fill Volume

25 mL

COA
COA

L1919

LB Agar Plates with Gentamicin-7, Kanamycin-50, Tetracycline-10, Bluo-gal-100, IPTG-40. 100mm, 20 Plates/Sleeve, Sterile.

Quantity

Minimum is set to 4 for this product*

scroll

Description
Formulation
Common Applications
Storage

LB Agar Plates with Gentamicin-7, Kanamycin-50, Tetracycline-10, Bluo-gal-100, IPTG-40 are nutrient agar plates formulated for the growth and selection of antibiotic-resistant E. coli strains. The LB base supports routine cultivation, and the combined antibiotics enable selection of transformants carrying compatible gentamicin-, kanamycin-, or tetracycline-resistance markers.

Bluo-Gal and IPTG facilitate blue/white screening via lacZ α-complementation in suitable host strains, allowing visual discrimination of colonies with functional versus disrupted β-galactosidase activity when using appropriate cloning vectors in E. coli.

  • 1.0% Tryptone
  • 0.5% Yeast Extract
  • 1.0% Sodium Chloride
  • 1.5% Agar
  • 50µg/mL Kanamycin Sulfate
  • 7µg/mL Gentamicin Sulfate
  • 10µg/mL Tetracycline Hydrochloride
  • 100µg/mL Bluo-Gal
  • 40µg/mL Isopropyl-B-D-thiogalactopyranoside(IPTG)

  • Triple-Antibiotic Selection: Simultaneous selection of transformants carrying kanamycin-, gentamicin-, and tetracycline-resistance plasmids in E. coli.

  • Co-Transformation/Co-Maintenance: Stable maintenance of multiple compatible plasmids with distinct resistance markers in E. coli.

  • Blue/White Screening: lacZ α-complementation screening using Bluo-Gal and IPTG to distinguish recombinant (white) from non-recombinant (blue) colonies in E. coli.

  • Reporter-Based Library Screening: Visual screening of clone libraries where lacZ readout (Bluo-Gal + IPTG) and triple selection are required in E. coli.

  • Expression Construct Screening: Plate-level IPTG induction to assess phenotype or reporter activation from lac-promoter plasmids under triple-antibiotic pressure in E. coli.

  • Bacterial Two-Hybrid/Interaction Assays: Selection of multi-plasmid systems with lacZ reporters for blue colony readout in E. coli.

  • Strain and Plasmid Verification: Replica plating and confirmation of resistance profiles and insert presence via blue/white outcomes in E. coli.

  • Colony Isolation and Picking: Single-colony isolation, CFU enumeration, and downstream plasmid prep workflows under stringent selection in E. coli.

  • Stability Testing: Assessment of plasmid retention and compatibility under combined kanamycin, gentamicin, and tetracycline selection in E. coli.

  • Contamination Control: Suppression of non-resistant contaminants by triple-antibiotic selection while enabling blue/white screening in E. coli.

Storage Temperature
Refrigerated
Shelf Life
60 days
Shows related citations for this product
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA
We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button