Blue-White Screening in Escherichia coli: X-Gal and IPTG enable lacZ α-complementation for rapid identification of recombinant colonies.

Triple Antibiotic Selection: Simultaneous selection for kanamycin (50 µg/mL), gentamicin (7 µg/mL), and tetracycline (10 µg/mL) resistance markers.

Co-transformation and Multi-plasmid Maintenance: Supports growth of Escherichia coli strains harboring multiple plasmids with distinct resistance cassettes.

Clone Screening after Ligation/Gibson/Golden Gate: Efficient identification and picking of correct transformants using colorimetric and antibiotic selection.

Library Screening and Directed Evolution: Differentiation of variants via blue-white screening under combined antibiotic pressure.

Strain Validation and Stability Testing: Confirms retention of multiple resistance markers across passages in Escherichia coli.

Replica Plating: Parallel analysis of colony phenotypes and plasmid combinations on selective media.

Plasmid Maintenance for Expression Workflows: Ensures stable propagation of helper, expression, and accessory plasmids in Escherichia coli.

Screening of Complementation Constructs: Uses IPTG induction to assess lac-based reporters and regulatory elements in Escherichia coli.

Contamination Control: Suppresses growth of non-resistant background bacteria via triple antibiotic selection.