Blue-White Screening: Differentiates recombinant from non-recombinant colonies via lacZ α-complementation in E. coli using X-Gal and IPTG.

Antibiotic Selection: Selects and maintains plasmids bearing beta-lactam resistance in E. coli using carbenicillin.

Clone Library Screening: Screens ligation, Gibson Assembly, TA, or Golden Gate cloning libraries for inserts in E. coli.

Colony Picking and Verification: Enables efficient picking of white colonies for downstream colony PCR, restriction analysis, or sequencing.

Transformation Efficiency Assessment: Measures competent cell performance by CFU counts and blue/white ratios in E. coli.

Reporter and Promoter Testing: Assesses lac-based and IPTG-inducible promoter activity on plates in E. coli.

Plasmid Maintenance and Stability: Propagates and preserves plasmids with ampicillin-class selection in E. coli.

Strain Validation for α-Complementation: Verifies compatibility of host strains (e.g., lacZΔM15) and vectors carrying lacZα in E. coli.