Selection of Chloramphenicol-Resistant Clones: Isolates colonies carrying cat-marked plasmids or chromosomal integrations at 34 µg/mL chloramphenicol.

Bacterial Transformation and Cloning: Plates transformants on LB agar with 1% glucose to recover and select recombinant clones.

Plasmid Maintenance: Maintains cat-selectable plasmids in hosts such as Escherichia coli, Salmonella enterica, and Bacillus subtilis.

Recombinant Protein Workflows: Supports strain upkeep for expression vectors with chloramphenicol resistance.

Catabolite Repression (1% Glucose): Reduces basal expression from inducible promoters to stabilize clones carrying toxic or burdensome inserts.

Library Construction and Screening: Enables selection of mutant or genomic libraries under chloramphenicol pressure.

CFU Enumeration on Selective Media: Quantifies viable, resistant bacteria in mixed populations.

Genetic Exchange Selection: Selects recipients after conjugation, transduction, or recombineering using cat markers.

Chromosomal Integration Verification: Recovers integrants or allelic replacements with chloramphenicol cassettes.

Contamination Control: Restricts growth to chloramphenicol-resistant strains on LB agar containing 34 µg/mL chloramphenicol.