Antibiotic Selection of Transformants: Selects Escherichia coli harboring AmpR/CarbR plasmids using 75 µg/mL carbenicillin.

Cloning and Plasmid Maintenance: Supports propagation of beta-lactamase–bearing plasmids in E. coli with reduced satellite colonies versus ampicillin.

Transformation Workflows: Plating after chemical transformation or electroporation of E. coli to recover resistant colonies.

Toxic Gene Handling: 1% glucose represses catabolite-sensitive promoters (e.g., lac/tac/T7-lac) to reduce leaky expression during cloning in E. coli.

Pre-Induction Plasmid Propagation: Maintains T7/lac-based expression plasmids in E. coli prior to induction to improve clone stability.

Colony Isolation and Purification: Streaking for single colonies, replica plating, and CFU enumeration under carbenicillin selection.

Colony Screening: Picking colonies for mini-prep or colony PCR from selective plates.

Conjugation and Mating Selections: Selecting AmpR/CarbR transconjugants in E. coli on carbenicillin plates.

Blue-White Compatible Workflows: Suitable background for X-gal/IPTG–based screening with AmpR/CarbR plasmids in E. coli (reduced basal lac expression with 1% glucose).

General LB-Based Culturing: Routine growth on LB agar (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl, 1.5% agar) supplemented with 1.0% glucose and 75 µg/mL carbenicillin for E. coli.