Antibiotic Selection of Transformants: Selects for ampicillin-resistant plasmid-bearing Escherichia coli colonies using 100 µg/mL ampicillin.

Post-Transformation Plating: Recovers and isolates colonies after heat-shock or electroporation of E. coli with Amp^R plasmids.

Single-Colony Isolation: Streaking to single colonies for clonal purification of Amp^R strains.

Colony PCR Screening: Rapid verification of inserts or constructs directly from E. coli colonies.

Starter Culture Inoculation: Picking verified colonies to start liquid cultures for minipreps or expression.

CFU Enumeration with Selection: Quantifying viable Amp^R cells via colony counting.

Plasmid and Strain Maintenance: Short-term storage and routine passaging of ampicillin-resistant E. coli clones.

Reduced Basal Expression: 1% glucose suppresses lac/T7-lac promoter leakiness during cloning of potentially toxic genes in E. coli.

Replica Plating: Confirming antibiotic resistance and phenotype consistency of transformed E. coli colonies.

Library Construction and Screening: Selecting members of plasmid libraries requiring ampicillin resistance in E. coli.