Selective plating of AmpR transformants: Isolation of carbenicillin-resistant Escherichia coli carrying bla-encoded plasmids.

Plasmid maintenance and cloning: Stable propagation of beta-lactam–selected plasmids with reduced satellite colonies versus ampicillin.

Transformation recovery and CFU enumeration: Plating competent Escherichia coli post-transformation to quantify and recover resistant colonies.

Colony isolation and strain preservation: Streaking for single colonies and routine passaging of resistant Escherichia coli under selection.

Reduced basal expression during cloning: 1% glucose suppresses leaky expression from lac/T7 promoters when cloning potentially toxic inserts.

Screening prior to miniprep/sequencing: Antibiotic-based colony selection to enrich for plasmid-bearing clones.

Replica plating for plasmid retention: Assessing maintenance or loss of bla-marked plasmids across passages.

Conjugation/mating selection: Selecting bla-bearing transconjugants on carbenicillin-containing LB agar.