Selection of Spectinomycin-Resistant Transformants: Isolates colonies harboring aadA/spectinomycin-resistance plasmids on LB agar containing 50µg/mL spectinomycin.

Plasmid Propagation and Maintenance: Ensures stable maintenance of spectinomycin-selectable plasmids in Escherichia coli and other Gram-negative hosts.

Cloning and Strain Construction: Supports cloning workflows requiring spectinomycin selection for inserts, cassettes, or genome edits.

Streaking and Single-Colony Isolation: Enables colony purification, isolation of single colonies, and downstream colony PCR or sequencing.

Agrobacterium Workflows: Maintains spectinomycin-marked Agrobacterium tumefaciens strains for plant transformation and vector propagation.

Plasmid Retention and Stability Assays: Evaluates plasmid stability under spectinomycin selection across serial passages.

Validation of Resistance Cassettes: Screens and confirms functional aadA/spectinomycin resistance inserts via colony growth.

Animal-Free Microbial Culture: Supports regulatory or sensitive applications requiring animal-component-free LB (1.69% animal-free soytone, 0.50% yeast extract, 1.00% NaCl, 1.50% agar) with spectinomycin.