Antibiotic Selection of Chloramphenicol-Resistant Transformants: Selects E. coli carrying chloramphenicol-resistance markers/plasmids at 34 µg/mL after heat-shock or electroporation.

Plasmid Maintenance and Cloning: Maintains Cm^R plasmids (e.g., pACYC184, BAC/PAC/cosmid vectors) in E. coli under constant selection.

Colony Isolation and CFU Enumeration: Streaking for single colonies and counting CFUs under chloramphenicol selection on 100 mm plates.

Recombineering and Gene Editing Workflows: Selection of Cm^R insertion cassettes and recombinants in E. coli.

Conjugation and Transduction Selections: Recovery and selection of Cm^R transconjugants or transductants in E. coli.

Protein Expression Strain Maintenance: Maintenance of expression plasmids with Cm^R in E. coli between induction cycles.

Quality Control of Resistance and Plasmid Stability: Verifying retention of chloramphenicol-resistance markers and plasmids over passages.

Animal-Origin–Free Microbial Culture: Supports animal-component–free workflows using 1.69% animal-free soytone, 0.50% yeast extract, 1.00% NaCl, 1.50% agar, and 34 µg/mL chloramphenicol.