Bacterial Transformation Selection: Selects transformants carrying spectinomycin-resistance plasmids (e.g., aadA) in Escherichia coli and other Gram-negative bacteria using 50 µg/mL spectinomycin.

Plasmid Maintenance and Cloning: Maintains and propagates recombinant plasmids with spectinomycin markers during cloning workflows on LB (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl, 1.5% agar).

Colony Screening: Enables colony picking, colony PCR, and sequencing prep from selective colonies to verify inserts and plasmid integrity.

CRISPR/Cas Workflows: Selects cells harboring genome-editing plasmids bearing spectinomycin resistance for editing and curing steps.

Conjugation/Mobilization Selection: Recovers transconjugants after biparental/triparental mating in organisms such as Escherichia coli, Agrobacterium tumefaciens, and Pseudomonas putida.

Expression Strain Propagation: Grows and maintains recombinant expression strains carrying spectinomycin-selectable vectors prior to induction.

Strain Preservation/Streaking: Streaks for single colonies and short-term storage while preserving plasmid selection pressure.

Resistance Verification: Confirms spectinomycin resistance phenotypes and monitors plasmid retention in laboratory strains.