Plasmid Selection and Maintenance: Selects and maintains strains harboring kanamycin- and streptomycin-resistance plasmids in Escherichia coli using dual antibiotic pressure (50 µg/mL each).

Dual-Marker Co-Selection: Ensures retention of multi-plasmid systems or bicistronic constructs requiring both kanamycin and streptomycin resistance.

Clonal Isolation and Colony Purification: Streaks for single colonies on 1.5% agar LB to obtain pure, antibiotic-selected clones.

Transformation and Library Screening: Screens transformants or pooled libraries by colony growth under Kan50/Str50 selection.

Replica Plating and Stability Testing: Assesses plasmid stability and phenotype retention via replica plating on dual-antibiotic LB agar.

Conjugation/Mating Selection: Selects transconjugants after bacterial mating (e.g., Escherichia coli donor/recipient workflows) using dual resistance markers.

CRISPR and Genome Engineering Workflows: Maintains editing vectors and helper plasmids requiring kanamycin and streptomycin resistance.

Contamination Control in Cultures: Enriches for intended resistant strains while suppressing susceptible contaminants on solid LB medium.