Dual-Antibiotic Plasmid Selection: Selects transformants carrying carbenicillin- and spectinomycin-resistance markers on LB agar, typically in Escherichia coli.

Cloning and Transformant Screening: Plates post-ligation or assembly reactions to recover colonies with correctly assembled vectors bearing both resistance cassettes.

Co-transformation and Co-maintenance: Ensures retention of two compatible plasmids by simultaneous selection with Carbenicillin-100 and Spectinomycin-50.

Colony PCR Template Preparation: Isolates single colonies from selective plates for rapid colony PCR verification of inserts.

Protein Expression Strain Selection: Confirms uptake of expression and helper plasmids before induction in E. coli production strains.

CRISPR/Cas Vector Selection in Bacteria: Recovers colonies carrying CRISPR/Cas and guide plasmids requiring dual antibiotic selection.

Plasmid Library Construction and Amplification: Plates combinatorial or mutagenesis libraries while maintaining dual-selectable backbones.

Conjugation/Mating Selection: Selects recipients that acquired dual-resistance plasmids after bacterial mating experiments.

Stability and Segregational Maintenance Testing: Monitors plasmid retention under dual selection across passages.

Archival Streaking and Culture Maintenance: Streaks glycerol stocks or mixed cultures to isolate and preserve dual-resistant clones.