Dual-Antibiotic Selection of Plasmids: Selects transformants in Escherichia coli carrying vectors with bla (carbenicillin/ampicillin) and nptII/aph(3') (kanamycin) resistance markers.

Co-Transformation and Co-Maintenance: Maintains two compatible plasmids in Escherichia coli by simultaneous selection at 100 µg/mL carbenicillin and 50 µg/mL kanamycin.

Protein Expression Systems: Supports dual-marker expression workflows (e.g., vector + helper/plasmid) in Escherichia coli under LB Miller conditions (1.0% NaCl, 1.5% agar).

Cloning and Subcloning Workflows: Enables recovery and maintenance of recombinant clones post-ligation or Gibson/Golden Gate assembly with dual resistance selection.

Library Construction and Screening: Facilitates selection and replica plating of dual-antibiotic plasmid libraries in Escherichia coli.

Colony Isolation and Picking: Provides sterile 100 mm plates for streaking, single-colony isolation, and clonal purification under combined selection.

CFU Enumeration Under Selection: Quantifies transformation efficiency and plasmid stability by plating serial dilutions with both antibiotics.

Strain and Vector Stability Testing: Assesses retention of dual-resistance plasmids during passaging in Escherichia coli and other Gram-negative hosts such as Pseudomonas aeruginosa.

Conjugation and Mating Selections: Selects recipients or transconjugants requiring both carbenicillin and kanamycin resistance markers.

Shuttle Vector Maintenance: Maintains dual-marker shuttle plasmids in Escherichia coli prior to downstream transfer to other hosts.