Dual-Antibiotic Selection of Transformants: Selects E. coli carrying plasmids with bla (AmpR; 100 µg/mL carbenicillin) and cat (CmR; 12.5 µg/mL chloramphenicol).

Co-Transformation and Co-Maintenance: Maintains E. coli strains harboring two compatible plasmids requiring AmpR and CmR.

BAC and Low-Copy Plasmid Propagation: Supports growth of cat-marked BACs/low-copy vectors at 12.5 µg/mL chloramphenicol.

Clone Isolation and Colony Picking: Plates transformation mixtures for isolating single colonies under dual selection.

Colony PCR Screening: Provides selective growth for picking colonies for downstream PCR verification.

Plasmid Library Plating: Plates combinatorial or genomic libraries requiring AmpR + CmR selection.

Protein Co-Expression Workflows: Selects E. coli expression strains carrying multiple vectors with AmpR and CmR markers.

Strain Preservation: Ensures selective pressure when picking colonies for glycerol stocks and master plates.