Bacterial Transformation and Selection: Selects Escherichia coli transformants carrying bla (carbenicillin) and cat (chloramphenicol) resistance markers.

Dual-Plasmid Maintenance: Maintains co-transformed plasmids in E. coli requiring simultaneous carbenicillin and chloramphenicol selection.

Cloning and Subcloning: Enables colony isolation and purification of constructs bearing AmpR and CamR markers in E. coli.

Library Screening: Screens combinatorial or mutagenesis libraries under dual-antibiotic pressure for enriched variants.

Genome Editing Workflows: Supports selection of CRISPR/editing plasmids with bla and cat markers during strain engineering in E. coli.

Recombinant Protein Expression Setup: Starts expression cultures from single colonies harboring AmpR/CamR plasmids.

BAC/PAC/Cosmid Maintenance: Maintains chloramphenicol-marked large constructs alongside helper plasmids with bla in E. coli.

Plasmid Stability Assays: Assesses segregational stability and retention of dual-marker plasmids over passages.

Colony Screening: Provides isolated colonies for PCR, sequencing, or reporter-based verification under dual selection.

Glycerol Stock Preparation: Generates long-term stocks from single, dual-selected E. coli colonies.