Dual-Antibiotic Plasmid Selection: Selects transformants carrying chloramphenicol- and kanamycin-resistant plasmids in E. coli.

Co-Transformation Screening: Identifies colonies that have successfully taken up two compatible plasmids (Cam^R and Kan^R).

Plasmid Maintenance: Maintains dual-plasmid systems (e.g., expression vector + helper/regulatory plasmid) under stringent selection.

Library Construction and Cloning: Screens recombinant libraries where vectors require simultaneous chloramphenicol and kanamycin resistance.

Conjugation/Mobilization Assays: Selects recipients bearing both resistance markers after mating in E. coli or Agrobacterium tumefaciens.

Transposon/Genomic Integration Studies: Recovers strains with combined genomic and plasmid markers conferring Cam^R and Kan^R.

Protein Expression Workflows: Plates expression strains carrying a Kan^R expression plasmid and a Cam^R chaperone/regulatory plasmid.

Contamination Control: Reduces growth of unwanted microbes by requiring dual resistance while culturing engineered E. coli.

Synthetic Biology Constructs: Maintains multi-plasmid circuits with distinct selection markers for stable operation.

Strain Verification: Confirms retention of both antibiotic markers during strain banking and passaging.