Dual-Antibiotic Selection: Selection and maintenance of plasmids encoding AmpR (bla) and CamR (cat) in E. coli under simultaneous ampicillin (100 µg/mL) and chloramphenicol (34 µg/mL) pressure.

Co-Transformation Workflows: Isolation of E. coli transformants harboring two compatible plasmids, each with AmpR or CamR.

Streaking for Single Colonies: Purification of dual-resistant E. coli clones for downstream cloning and expression.

Post-Transformation Plating: Recovery and selection of heat-shock or electroporated E. coli on dual-selection plates.

Colony Screening: Picking dual-resistant colonies for colony PCR, miniprep, and restriction analysis.

Plasmid Stability Maintenance: Continuous culture of E. coli strains to enforce retention of AmpR/CamR plasmids.

Library Construction and Maintenance: Selection of genomic or cDNA libraries in E. coli requiring AmpR and CamR.

Replica Plating: Verification of dual-resistance profiles and plasmid combinations in E. coli.

Co-Expression Systems: Maintenance of helper and expression plasmids (e.g., T7 systems) in E. coli with dual selection.

Conjugation/Mating Selections: Isolation of E. coli transconjugants that acquire both AmpR and CamR markers.