Dual-Antibiotic Selection of Transformants: Isolates colonies carrying chloramphenicol- and kanamycin-resistance plasmids in E. coli.

Co-Transformation and Multi-Plasmid Maintenance: Maintains compatible plasmid systems (e.g., expression + helper) under combined selection in E. coli.

Cloning Workflow Screening: Selects correct constructs after Gibson/Golden Gate cloning using dual resistance markers.

Genome Editing and Recombineering: Selects edits incorporating chloramphenicol/kanamycin cassettes or dual-marker landing pads.

Colony Isolation and CFU Enumeration: Purifies colonies and quantifies transformation efficiency under dual selection.

Plasmid Stability Assays: Evaluates segregational and structural stability of dual-marker plasmids across passages.

Conjugation and Mobilization Selection: Recovers transconjugants bearing chloramphenicol/kanamycin markers.

Transposon Mutagenesis Screens: Selects insertions using Cm/Kan markers and enables combinatorial selections.

Library Maintenance and Screening: Sustains and screens variant libraries requiring dual antibiotic pressure.

Strain Archiving and Verification: Maintains and confirms identity of strains carrying Cm/Kan resistance in E. coli.