Dual-Antibiotic Selection of Escherichia coli Transformants: Selects colonies carrying chloramphenicol- and kanamycin-resistance markers (25 µg/mL Cm, 50 µg/mL Kan) on LB agar.

Maintenance of Dual-Selectable Plasmids: Preserves plasmids or constructs requiring both cat and kan resistance in Escherichia coli.

Two-Plasmid System Support: Enables co-maintenance of compatible vectors (e.g., CRISPR/Cas and donor/helper plasmids) needing Cm and Kan selection.

Transformant Screening: Identifies correct co-transformants after chemical transformation or electroporation using dual selection.

Cloning Workflows: Supports subcloning and assembly steps when backbone and insert/helper vectors carry Cm and Kan markers.

Library Propagation: Maintains plasmid or genomic libraries under dual selection to minimize plasmid loss and background.

Protein Expression Strain Selection: Selects expression constructs and helper plasmids in Escherichia coli for stable protein production.

Bacterial Two-Hybrid and Interaction Assays: Supports systems that use chloramphenicol- and kanamycin-marked vectors for reporter readouts.

Conjugation/Co-Integration Selection: Recovers transconjugants or recombinants requiring simultaneous Cm and Kan resistance.

Quality Control of Plasmid Stability: Verifies retention of dual-resistance cassettes during passage on LB agar.