Dual-Antibiotic Plasmid Selection: Selects recombinant Escherichia coli carrying plasmids with bla (carbenicillin/ampicillin) and cat (chloramphenicol) resistance markers.

Co-Maintenance of Two Plasmids: Supports stable propagation of compatible plasmid pairs (bla+ and cat+) in E. coli cloning and expression strains.

BAC/Cosmid Workflows: Maintains cat-resistant BACs/cosmids alongside helper/accessory plasmids with bla selection in E. coli.

Post-Transformation Plating: Plates chemically competent or electrocompetent E. coli for colony isolation and selection under combined antibiotic pressure.

Colony Screening: Enables picking antibiotic-resistant colonies for downstream colony PCR, restriction analysis, or sequencing.

Strain Maintenance: Preserves engineered E. coli strains with integrated or plasmid-borne chloramphenicol and carbenicillin resistance cassettes.

Blue-White Screening (when supplemented): Supports alpha-complementation in E. coli when X-Gal/IPTG are added to the plate surface.

Starter Culture Isolation: Provides single colonies for inoculating liquid cultures for cloning, subcloning, or small-scale expression checks.