Dual-Antibiotic Plasmid Selection: Selects transformants carrying carbenicillin- and kanamycin-resistance plasmids in E. coli.

Co-Transformation and Dual-Plasmid Maintenance: Supports stable maintenance of two compatible plasmids requiring bla and nptII markers in E. coli.

Clone Isolation and Colony Screening: Enables picking of recombinant colonies for colony PCR or sequencing from E. coli plates.

Plasmid Propagation: Grows high-fidelity plasmid-bearing E. coli colonies for miniprep/maxiprep workflows.

Strain Construction and CRISPR Workflows: Selects edited E. coli carrying helper and donor plasmids with carbenicillin and kanamycin resistance.

Expression Vector Selection: Maintains selection for expression constructs prior to induction in E. coli hosts.

Transconjugant Selection: Plates conjugation/mating products requiring simultaneous carbenicillin and kanamycin selection in E. coli.

Glycerol Stock Recovery and Strain Verification: Recovers and verifies dual-selected E. coli strains from frozen stocks.