Dual-Antibiotic Selection: Selects transformed E. coli carrying ampicillin- and kanamycin-resistance plasmids on LB agar (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar; 100 µg/mL Ampicillin, 50 µg/mL Kanamycin).

Co-Transformation Maintenance: Maintains multiple compatible plasmids (e.g., helper and expression vectors) in E. coli under Amp/Kan dual selection.

Colony Isolation and Screening: Enables single-colony isolation for colony PCR, sequencing, and verification of recombinant E. coli with dual markers.

CRISPR/Recombineering Workflows: Supports stable propagation of CRISPR or recombineering plasmid systems requiring ampicillin and kanamycin selection in E. coli.

Starter Culture Preparation: Provides selective plates for picking verified colonies to inoculate liquid cultures for plasmid minipreps and expression.

Library Construction and Maintenance: Used for maintaining dual-marker plasmid libraries or combinatorial constructs in E. coli.

Strain Validation and QC: Confirms presence of Amp/Kan resistance markers to detect loss of plasmids or contamination in E. coli stocks.

Genetic Transfer Workflows: Selects desired recipients or transductants in E. coli after conjugation or transduction when Amp/Kan markers are employed.