Selection of Kanamycin-Resistant Transformants: Isolates colonies carrying kanR plasmids after heat-shock or electroporation in Escherichia coli.

Plasmid Maintenance and Propagation: Maintains selection pressure for stable inheritance of kanR vectors during streaking and passaging of Escherichia coli.

Cloning and Subcloning Workflows: Supports recovery and screening of recombinant clones bearing kanamycin-selectable plasmids.

Colony Screening and PCR Picking: Grows discrete colonies for downstream colony PCR, minipreps, and sequencing verification of kanR constructs.

CRISPR/Genome Editing Selections: Selects edited cells when kanamycin resistance is used as the selectable marker.

Transposon and Insertion Mutagenesis: Recovers mutants carrying kanamycin-marked insertions.

Conjugation and Mating Selections: Selects transconjugants bearing kanR plasmids or markers after bacterial mating.

Expression Strain Work: Propagates kanR expression vectors for protein production in hosts such as Escherichia coli.

Replica Plating and Stability Testing: Assesses plasmid retention and antibiotic dependence by replicating colonies onto kanamycin plates.

Contamination Control: Suppresses growth of non-resistant background flora while enriching target kanR strains (e.g., Escherichia coli lab strains).