Ampicillin-Resistant Transformant Selection: Plates Escherichia coli transformants carrying bla/ampR plasmids at 50 µg/mL ampicillin.

PBAD/araC-Induced Expression Screening: Enables robust plate-based induction of genes under the arabinose-inducible PBAD promoter (10 mM arabinose) in Escherichia coli.

Reporter Assays (e.g., GFP/RFP): Visual screening of PBAD-driven fluorescent or colorimetric reporters on colonies of Escherichia coli.

Plasmid Maintenance Under Selection: Sustains PBAD/araC plasmids with ampicillin selection during streaking, isolation, and storage of Escherichia coli strains.

Toxic Gene Expression on Plates: Induces controlled, colony-level expression of otherwise burdensome or toxic genes from PBAD in Escherichia coli.

Colony Screening and Picking: Rapid isolation of correctly transformed Escherichia coli colonies for downstream colony PCR, minipreps, and sequencing.

Recombineering/Genome Editing Workflows: Supports arabinose-inducible systems (e.g., PBAD-driven recombinases or editing components) during selection in Escherichia coli.

CRISPRi/CRISPRa Induction Tests: Plate-level induction and assessment of PBAD-controlled dCas9 or guide expression in Escherichia coli.