Antibiotic-Selective Cloning: Selection and isolation of transformants carrying tetracycline-resistance plasmids in E. coli (5 µg/mL tetracycline for efficient colony recovery).

Plasmid Maintenance: Stable propagation of tet-marked plasmids and genomic constructs in E. coli and Bacillus subtilis under antibiotic pressure.

Transformation and Efficiency Plating: Quantifying transformation efficiency and recovery of competent cells on tetracycline-selective LB agar.

Recombinant Screening: Colony screening (e.g., colony PCR or blue-white screening with IPTG/X-gal) for inserts on vectors conferring tetracycline resistance.

Strain Preservation: Preparing clonal isolates and glycerol stocks from single colonies while maintaining tet-linked genetic elements.

Conjugation/Transposition Selection: Selecting transconjugants or transposon insertion mutants with tet markers in E. coli and related Enterobacteriaceae.

Regulatory Circuit Assays: Evaluating TetR/TetA-based promoters and reporter constructs where tetracycline modulates transcriptional control.

Antibiotic Susceptibility/Resistance Studies: Monitoring tetracycline resistance phenotypes and stability in lab strains (e.g., E. coli, Pseudomonas aeruginosa).

Library Selections: Enrichment of barcoded or mutagenesis libraries carrying tetracycline-selectable elements.

Quality Control of Constructs: Verifying retention of tet-resistance cassettes in plasmids, BACs, or cosmids during subcloning and passaging.