Selection of Kanamycin-Resistant Transformants: Plates transformed Escherichia coli for recovery of colonies carrying kanamycin-resistance plasmids (30 µg/mL kanamycin sulfate).

Clonal Isolation and Colony Picking: Enables single-colony isolation and downstream expansion of kanamycin-resistant clones.

Plasmid Maintenance and Stability: Maintains selective pressure to preserve kan-bearing plasmids in E. coli during routine passaging.

CRISPR and Strain Engineering: Selects edited bacterial clones that incorporate kanamycin resistance cassettes.

CFU Enumeration Under Selection: Quantifies viable kanamycin-resistant cells for transformation efficiency and viability assays.

Conjugation/Mating Selection: Recovers transconjugants carrying kan-marked elements in E. coli or Agrobacterium tumefaciens.

Maintenance of Non-Enteric Gram-Negatives: Cultures kan-resistant strains such as Pseudomonas putida under selective conditions.

Animal-Free Microbial Culture: Supports growth with plant-derived peptones (animal-free soytone) for animal-origin–free workflows.