Plasmid Selection in E. coli: Selects for cells carrying gentamicin-resistance plasmids (10 µg/mL) on LB agar for stable maintenance.

Transformant Screening: Recovers and identifies successful heat-shock or electroporation transformants under gentamicin selection.

Clone Isolation and Single-Colony Purification: Streaks for single colonies to obtain clonal populations maintaining gentamicin-marked constructs.

CFU Enumeration Under Selection: Quantifies colony-forming units of gentamicin-resistant cells after dilutions or viability assays.

Conjugation and Transposon Mutagenesis Selection: Selects transconjugants or insertion mutants carrying gentamicin resistance in E. coli and related hosts.

Contamination Control on Selective Media: Suppresses gentamicin-sensitive contaminants while enriching resistant target strains.

Maintenance of Recombinant Strains: Short-term storage and passaging of engineered E. coli strains to retain gentamicin-marked plasmids.

Library Plating (Genomic, cDNA, or Transposon): Plates complex libraries to recover and expand clones with gentamicin-selectable markers.

Reporter/Expression Construct Maintenance: Ensures retention of gentamicin-marked reporter or expression vectors prior to liquid culture induction.

Resistance Phenotype Verification: Confirms gentamicin resistance at 10 µg/mL for strain or plasmid characterization.