Selection of Kanamycin-Resistant Transformants: Plating and isolating kanamycin-resistant Escherichia coli colonies after transformation, ligation, Gibson, or Golden Gate cloning.

Plasmid Maintenance: Routine culturing and passaging of strains carrying kanamycin-selectable plasmids to preserve construct integrity.

Single-Colony Isolation: Streaking for single colonies to ensure clonal purity under kanamycin selection.

Colony Screening: Picking colonies for downstream analyses such as colony PCR and Sanger sequencing.

CFU Enumeration: Quantifying viable kanamycin-resistant cells following transformation or selection workflows.

Genome Editing Selection: Selecting edited strains or allelic exchanges that use kanamycin resistance markers.

Transposon Mutagenesis: Selecting insertions from kanamycin-marked transposon libraries.

Bacterial Conjugation/Transduction Selection: Selecting recipients that acquire kanamycin-marked elements after conjugation or transduction (e.g., in Escherichia coli or Salmonella enterica).