Antibiotic Selection of Transformants: Selects Escherichia coli carrying AmpR/bla plasmids using 100 µg/mL carbenicillin.

Cloning and Subcloning Workflows: Screens recombinant colonies after ligation/transformation on LB agar.

Transformation Efficiency Assays: Quantifies colony-forming units by plating serial dilutions on selective media.

Plasmid Maintenance and Propagation: Streaks and passages single colonies of AmpR plasmid-bearing Escherichia coli.

Colony PCR Validation: Provides colonies for PCR screening to confirm inserts or edits.

CRISPR/Edit Selection in E. coli: Enriches edited clones maintained on AmpR plasmids in Escherichia coli.

Library Construction and Screening: Selects combinatorial plasmid libraries expressed in Escherichia coli.

Expression Vector Prescreening: Picks colonies harboring AmpR expression vectors for downstream culture.

Blue-White Screening (with Supplements): Enables α-complementation when supplemented with X-gal and IPTG.

Animal-Origin-Free Workflows: Supports AOF microbial selection where animal components are avoided.