Selection of Kanamycin-Resistant Transformants: Isolates colonies of Escherichia coli carrying plasmids with a kanamycin resistance marker (200 µg/mL) on LB agar (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar).

Plasmid Maintenance and Propagation: Sustains selection pressure to maintain kan-marked plasmids during routine culture of Escherichia coli.

Clone Screening After Ligation/Transformation: Enables colony isolation for downstream verification (colony PCR, restriction digest, sequencing) of kan-selected constructs.

Protein Expression Workflows: Supports growth of Escherichia coli harboring kan-marked expression vectors for recombinant protein production.

CRISPR/Cas and Genome Editing Selection: Selects edits or plasmid-borne CRISPR tools using kanamycin resistance in bacterial hosts such as Escherichia coli.

Strain Purification and Archiving: Streaks for single colonies and maintains purified kan-resistant strains on selective plates.

Conjugation and Mating Assays: Selects transconjugants using kan markers in systems involving Escherichia coli and other bacteria.

Transposon Mutagenesis Selection: Recovers mutants carrying kanamycin-tagged transposons in bacteria (e.g., Escherichia coli, Bacillus subtilis).

Library Construction and Screening: Grows pooled or arrayed libraries (e.g., sgRNA or mutant libraries) maintained by kan selection.

Colony PCR Template Preparation: Provides selective growth for picking colonies as DNA templates from kan-resistant clones.