Antibiotic Selection of Transformants: Selects Escherichia coli colonies harboring tetracycline-resistant plasmids at 12.5 µg/mL.

Plasmid Maintenance and Propagation: Maintains tet-marked plasmids (including low-copy/BAC vectors) in Escherichia coli under selective pressure.

Cloning and Colony Screening: Screens recombinant colonies carrying tetracycline-selectable vectors on LB agar.

Conjugation and Mobilization Assays: Selects tet-marked transconjugants after bacterial mating experiments.

CRISPR/Genome Editing Workflows: Selects cells retaining tet-selectable editing or donor plasmids during editing cycles.

Strain Maintenance and Streaking: Preserves strains with chromosomal tet-resistance markers by routine streaking and passaging.

Replica Plating and Verification: Confirms tetracycline resistance phenotypes and plasmid retention by replica plating.

Shuttle Vector Propagation: Supports tet-marked shuttle vectors used between Escherichia coli and Bacillus subtilis.

Agrobacterium Plasmid Selection: Selects tet-resistant plasmids in Agrobacterium tumefaciens on LB when appropriate.

Library Amplification: Amplifies pooled plasmid libraries (e.g., genomic or shRNA) using tet selection to maintain diversity.