Apramycin-Resistant Clone Selection: Selects and maintains plasmids or genomic integrations carrying aac(3)IV in Escherichia coli and other Gram-negative bacteria at 50 µg/mL.

Transformation Screening: Plates and screens recombinant transformants in E. coli using apramycin selection on LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar).

Intergeneric Conjugation Selection: Recovers apramycin-resistant exconjugants in Streptomyces following mating with E. coli donors.

Library Maintenance: Maintains BAC/cosmid/fosmid libraries with apramycin resistance markers in E. coli.

Genome Editing Selection: Selects CRISPR/Cas or recombineering edits that use apramycin resistance cassettes.

Allelic Exchange and Transduction: Selects marker exchange events or P1 transductants carrying apramycin resistance in E. coli.

Transposon Mutagenesis: Recovers mutants from Tn-based insertions with apramycin markers in E. coli and Pseudomonas.

Strain Authentication and Stability: Verifies plasmid retention and antibiotic resistance stability by streaking and colony isolation.

CFU Enumeration Under Selection: Quantifies viable counts of apramycin-resistant cells from mixed cultures.

Pathogen and Commensal Workflows: Selects engineered strains of Salmonella, Klebsiella, and other Enterobacteriaceae carrying apramycin markers.